Umuyoboro wa CPT 8ml

Vacutainer CPT (Cell Preparation Tube) 8mL numuyoboro wihariye wo gukusanya amaraso ukoreshwa mugutandukanya ingirabuzimafatizo (PBMCs) mumaraso yose. Bikunze gukoreshwa mu gukingira indwara, ubushakashatsi ku ndwara zandura, no mu mavuriro nko gukurikirana ubudahangarwa bw'umubiri hamwe n'ubushakashatsi bwakozwe.

Ibintu by'ingenzi biranga Vacutainer CPT Tube (8mL):
Byuzuye byuzuye anticoagulant hamwe nubucucike buringaniye
Harimo Sodium Citrate nka anticoagulant.
Harimo Ficoll ™ -bishingiye kuri density gradient gutandukanya gel kugirango byorohereze gutandukanya ingirabuzimafatizo.
Gushoboza intambwe imwe PBMC kwigunga
Kurandura ibikenerwa byintoki gakondo ya centrifugation (urugero, Ficoll-Paque ™).
Kugabanya igihe cyo gutunganya no guhinduka.
Ubushobozi bwamaraso asanzwe
Yagenewe gutunganya kugeza kuri 8mL yamaraso yose.
Sterile, umuyoboro umwe
Byakoreshejwe mubuvuzi nubushakashatsi aho bisabwa ubuziranenge bwa PBMC.
Bihujwe na centrifugation
Porotokole yihariye ya centrifugation isabwa gutandukanya neza PBMCs nibindi bice bigize amaraso.

Imikoreshereze na Porogaramu:
Kwigunga kwa PBMC kubushakashatsi bwimikorere yubudahangarwa (T selile, B selile, NK selile, monocytes).
Flow cytometrie, ELISPOT, cytokine, hamwe nisesengura ryikingira.
Indwara zandura n'ubushakashatsi bw'inkingo.
Gukurikirana indwara ya Autoimmune.
Igeragezwa rya Clinical no kuvumbura biomarker.

Uburyo Bikora (Inzira Yibanze):
1. Kusanya icyitegererezo cyamaraso mumiyoboro ya CPT (kuzuza ingano yagenwe).
2. Hindura witonze (inshuro 8-10) kugirango uvange na anticoagulant.
3. Centrifuge nkuko protocole yabayikoze (mubisanzwe hafi 1500-1800 RCF muminota 20-30 mubushyuhe bwicyumba).
4. PBMC igaragara hejuru ya gel itandukanya nyuma ya centrifugation.
5. Kuramo witonze gukuramo PBMC ukoresheje umuyoboro wa porogaramu yo hasi.

Kubika no Gukemura:
Bika ku cyumba cy'ubushyuhe (15-25 ° C).
Irinde ubushyuhe bukabije cyangwa ubukonje.